Bovine and feline Tritrichomonas foetus total cellular protein mass spectrometry data files

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General
Title
Bovine and feline Tritrichomonas foetus total cellular protein mass spectrometry data files
Type
Dataset
Date Record Created
2016-03-23
Date Record Modified
Not provided
Language
English
Embargo
Embargo
Not embargoed.
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Embargo comment
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Coverage
Date Coverage
2014-03-07 to 2014-03-20
Time Period
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Geospatial Location
Not provided
Description
  1. This is the raw data from Mass Spectrometry analysis of spots resolved using two-dimensional analysis from a total protein sample from Tritrichomonas foetus bovine and feline genotypes. The dataset contains data files generated following the analysis of protein spots using LC-MS/MS. There are two types of data files. .Wiff files were produced using the following procedure: LC−MS/MS was performed using a QSTAR Elite hybrid Q-TOF mass spectrometer (Applied Biosystems, Sciex, USA). The peptides were washed off the trap at 300 nL min-1 onto a PicoFrit column (75 μm × 100 mm) (New Objective, USA) packed with Magic C18AQ resin (Michrom Bioresources, USA), and the following protocol was used to elute peptides from the column and into the source of a QSTAR Elite hybrid Q-TOF mass spectrometer (Applied Biosystems, Sciex, USA): 5−30% MS buffer B (98% acetonitrile−0.2% formic acid) over 8 minutes, 30−80% MS buffer B over 3 minutes, 80% MS buffer B for 2 minutes, 80−85% MS buffer B for 3 minutes. MS/MS fragmentation ion scans were calibrated using fragments of Glu-Fibrinopeptide B. MS/MS spectral data was converted to Mascot Generic format using TOF/TOF extractor V2.1 (Michigan Proteome Consortium, 2003). .raw files were produced using the following procedure: LC-MS/MS analysis was undertaken using a nanoAcquity UPLC (Waters, USA) coupled to a XevoQToF (Waters, USA) mass spectrometer. The peptides were washed off the trap at 400 nL/min on to a C18 BEH analytical column (75 μm ´ 100 mm)(Waters, USA), packed with 1.7 μm particles of pore size 130 Å and the following protocol was used to elute peptides from the column into the source of a XevoQToF mass spectrometer (Waters, USA): 1-50% MS buffer B (98% acetonitrile, 0.2% formic acid) over 30 minutes, 50-85% MS Buffer B over 2 minutes, 85% MS buffer B 3 minutes, 85-99% over 1 minute. After separation, the peptides were analysed using tandem mass spectrometry, implementing an emitter tip that tapers to 10 µm at 2300 V. A Data Directed Acquisition (DDA) experiment was performed which continuously scanned for peptides of charge state 2+ to 4+ with an intensity of more than 50 counts per second, with a maximum of three ions in any given 3 second scan. The data can be accessed via the URL in the Rights section of this record.

    • Type: full
Related Publications
  1. Stroud, L. J., Slapeta, J., Padula, M. P., Druery, D., Tsiotsioras, G., Coorssen, J. R., & Stack, C. M. (2017). Comparative proteomic analysis of two pathogenic Tritrichomonas foetus genotypes : there is more to the proteome than meets the eye. International Journal for Parasitology. doi:10.1016/j.ijpara.2016.11.004
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Technical metadata
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People
Creators
  1. Ms Leah Stroud , School of Science and Health [PI]
Primary Contact
Colin Stack, c.stack@westernsydney.edu.au
Supervisors
Not provided
Subject
Fields of Research
  1. 0707 - Veterinary Sciences (0707)
Socio-Economic Objective
Not provided
Keywords
  1. LC-MS/MS data
  2. Protozoan parasites
  3. host adaptation
  4. two-dimensional electrophoresis
  5. comparative proteomics
Research Activity
Pure basic research
Rights
License
CC BY: Attribution 3.0 AU
License - Other
Not provided
Rights
Copyright Western Sydney University
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Citation
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Identifier Type
useCuration
Creators
  1. Ms Leah Stroud
  2. Dr Matthew Padula
Title
Bovine and feline Tritrichomonas foetus total cellular protein mass spectrometry data files
Edition
Not provided
Publisher
Western Sydney University
Place of Publication
Sydney
Dates
  1. 2016 {missing: "dc:type.skos:prefLabel" }
URL
http://doi.org/10.4225/35/5731634ffa723
Context
Stroud, Leah; Padula, Matthew (2016): Bovine and feline Tritrichomonas foetus total cellular protein mass spectrometry data files . Western Sydney University. http://doi.org/10.4225/35/5731634ffa723