Transcriptomic analysis of diverse isolates of Pisolithus sp. colonising Eucalyptus grandis under different competition scenarios

Details

Print this Page Print this Page
Show all sections
General
Title
Transcriptomic analysis of diverse isolates of Pisolithus sp. colonising Eucalyptus grandis under different competition scenarios
Type
Dataset
Date Record Created
2017-05-02
Date Record Modified
Not provided
Language
English
Embargo
Embargo
Not embargoed.
Embargo date
Not provided
Embargo comment
Not provided
Coverage
Date Coverage
Not provided
Time Period
Not provided
Geospatial Location
  1. Text:
  2. Text: POINT(150.74573636057 -33.611928273272)
Description
  1. Multiple ectomycorrhizal fungi (EMF) compete to colonise the roots of a host plant but it is not known whether their success is under plant or fungal control, or a combination of both. Using RNAseq, we assessed whether plants control EMF colonisation by preferentially allocating more carbon (C) to more beneficial partners in terms of nitrogen (N) supply or if other factors drive competitive success. We characterised coordination of nutrient exchange between the plant host Eucalyptus grandis and three Pisolithus isolates when growing alone and when competing either indirectly (with a physical barrier) or directly. Differences in nutrient exchange among isolates were related to differences in expression of key fungal and plant N and C transporter genes. When given a choice of partners, the plant was able to limit colonisation by the least cooperative isolate. This was not explained by a reduction in allocated C. Instead, our results suggest that partner choice in EMF could operate through the up-regulation of defence-related genes against those fungi providing fewer nutrients. \nThis dataset contains approximately 100 subsample folders of RNAseq fastq data. Each sample data comes complete with a matching CSV file of metadata. In total, the dataset volume equates to 150GB.

    • Type: full
Related Publications
Not provided
Related Websites
  1. Download HIE data
  2. HIE | Hawkesbury Institute for the Environment
Related Data
Not provided
Related Services
Not provided
Technical metadata
Not provided
People
Creators
  1. Jonathan Plett , Hawkesbury Institute for the Environment (HIE) [CI]
  2. Sara Hortal Botifoll , Hawkesbury Institute for the Environment (HIE)
  3. Krista Plett , Hawkesbury Institute for the Environment (HIE)
  4. Ian Anderson , Hawkesbury Institute for the Environment (HIE)
Primary Contact
Jonathan Plett, j.plett@westernsydney.edu.au
Supervisors
Not provided
Subject
Fields of Research
  1. 060505 - Mycology (060505)
  2. 050303 - Soil Biology (050303)
Socio-Economic Objective
Not provided
Keywords
  1. Multiple ectomycorrhizal fungi
  2. RNAseq
  3. Nutrient exchange
Research Activity
Not provided
Rights
License
CC BY 4.0: Attribution 4.0 International
License - Other
Not provided
Access Rights/Conditions
Not provided
Type
open
Rights
Not provided
Attachments
No files currently attached.
Citation
Sending Citation
on
Identifier Type
useCuration
Creators
  1. Jonathan Plett
  2. Sara Hortal Botifoll
  3. Krista Plett
  4. Ian Anderson
Title
Transcriptomic analysis of diverse isolates of Pisolithus sp. colonising Eucalyptus grandis under different competition scenarios
Edition
Not provided
Publisher
Western Sydney University
Place of Publication
Not provided
Dates
  1. 2017-05-02 {missing: "dc:type.skos:prefLabel" }
URL
http://doi.org/10.4225/35/590a6591427fc
Context
Plett, Jonathan; Hortal Botifoll, Sara; Plett, Krista; Anderson, Ian (2017): Transcriptomic analysis of diverse isolates of Pisolithus sp. colonising Eucalyptus grandis under different competition scenarios. Western Sydney University. http://doi.org/10.4225/35/590a6591427fc