Bacterial microbiome of four populations of Mycopsylla fici using 16S rDNA amplicon sequencing
  • Description

    The goals of this experiment are to assess the bacterial diversity associated with Mycopsylla fici (Psylloidea: Homotomidae), and to compare bacterial diversity and relative abundance between populations within the native and colonised range of this species. Insects were collected on Ficus macrophylla from four locations: Brisbane, Sydney and Lord Howe Island in Australia and Auckland in New Zealand between September 2013 and December 2014. Regions v3 and v4 of the 16S rDNA were amplified using the primers 341F (5’-CCTACGGGNGGCWGCAG) and 805R (5’-GACTACHVGGGTATCTAATCC) and sequenced (paired end, 2x300bp) on a Miseq platform (Next Generation Sequencing facility, Western Sydney University). When unzipped, the folder contains 96 fastq files and one Excel file with description of the insects used in the experiment.
    • - Start
      Data publication title Bacterial microbiome of four populations of Mycopsylla fici using 16S rDNA amplicon sequencing
    • Description

      The goals of this experiment are to assess the bacterial diversity associated with Mycopsylla fici (Psylloidea: Homotomidae), and to compare bacterial diversity and relative abundance between populations within the native and colonised range of this species. Insects were collected on Ficus macrophylla from four locations: Brisbane, Sydney and Lord Howe Island in Australia and Auckland in New Zealand between September 2013 and December 2014. Regions v3 and v4 of the 16S rDNA were amplified using the primers 341F (5’-CCTACGGGNGGCWGCAG) and 805R (5’-GACTACHVGGGTATCTAATCC) and sequenced (paired end, 2x300bp) on a Miseq platform (Next Generation Sequencing facility, Western Sydney University). When unzipped, the folder contains 96 fastq files and one Excel file with description of the insects used in the experiment.
      • Data type dataset
    • Keywords
      • Endosymbiont
      • Microbiome
      • Carsonella
      • Wolbachia
      • Psyllid
    • Funding source
    • Grant number(s)
      • -
      • FoR codes
      • 31 - BIOLOGICAL SCIENCES
      • 3103 - Ecology
      • 310302 - Community ecology (excl. invasive species ecology)
      • 3107 - Microbiology
      • 310703 - Microbial ecology
      SEO codes
    - Coverage
      Temporal (time) coverage
    • Start date 2013/09/01
    • End date 2015/07/01
      • Time period
       
      Spatial (location,mapping) coverage
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    - Data
    - Supplements
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    - Citation
      Citation Fromont, Caroline; Cook, James; Riegler, Markus; Sutton, Tim; Quintans, Desi; Hall, Aidan; DeGabriel, Jane (2017): Bacterial microbiome of four populations of Mycopsylla fici using 16S rDNA amplicon sequencing. Western Sydney University.

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